LABS

LINKED LABS AND SKILL REQUIREMENTS

The following labs are linked to the Skills Center and will consider student projects leading towards a honors degree. Requirements for joining a Skills Center linked lab is the completion and certification of all the lab required skills listed below for each individual lab. Once a student has completed the required skills the Skills Center proctors will work with the student and the targeted linked lab to design an independent research project (MCDB 4567) to be completed primarily in the Skills Center. Successful completion of an independent project helps to make students eligible to enter into an Honors Thesis project within the targeted linked lab.

ARNOULT LAB

Bacterial Transformation, Buffers and stock solutions, Crispr/Cas9, DNA Agarose Gels, DNA Mini-Prep, FACS, Fluorescence Microscopy, Immunoblot , IP, PCR

AYDIN LAB

Macromolecule Structure Analysis with Chimera, Protein Data Bank (PDB) 1, Protein Data Bank (PDB) 2, Uniprot Database, Electron Microscopy 1, Gene Cloning, PCR, Protein Expression, Protein Purification 3 (AFFI), Protein Purification 2 (SEC)

BETTERTON LAB

Introduction to Chimera, Macromolecule Structure Analysis with Chimera, Autoclave LB/Agar Sterilization, Bacterial Transformation, Buffers and stock solutions, Gene Cloning, Light Microscopy 3 (Fluorescence), PCR, Pipette Operation and Calibration, SDS-PAGE

CECH LAB

Protein Data Bank (PDB) 1, Bacterial Transformation, Buffers and stock solutions, Centrifugation 1, DNA Agarose Gels, DNA Mini-Prep, western blot, PCR, Pipette Operation and Calibration, SDS-PAGE

KLYMKOWSKY LAB

Crispr/Cas9, PCR. Cloning, Immunoprecipitation, EM,
Cell culture, BLAST
Tissue Culture

LUGER LAB

PCR, Protein Expression, Protein Purification, EM, Cloning, Immunoblot, qPCR, bacterial culture, SDS-PAGE, DNA purification, western blot, protein binding assays, PDB, Uniprot

SHEN LAB

PCR, Protein Purification, Centrifugation, Cloning, FACS, DNA Agarose gels

STOWELL LAB

PCR, Protein Expression, Protein Purification, EM, Cloning, western blot, qPCR, Chimera, Allen Brain Atlas, Chimera, PDB

THINK BIOSCIENCE

PCR. Cloning, Western Blot Immunoprecipitation, Protein Purification, Cell Culture

BIOLOOMICS

Protein Expression, Protein Purification, Cloning, Immunoblot, qPCR, bacterial culture, SDS-PAGE, western blot, protein binding assays

VORTEX BIOTECHNOLOGY

Cloning, bacterial transformation, protein expression, SDS-PAGE, western blotting, protein purification, solutions and media preparation, tissue culture, plate reader assays, PDB, ChimeraX

AMIDEBIO

Cloning, bacterial transformation, protein expression, SDS-PAGE, ITC, MST, protein purification, tissue culture, protein quantification, PDB, Chimera