LABS

LINKED LABS AND SKILL REQUIREMENTS

The following labs are linked to the SkillsCenter and will consider student projects leading towards a honors degree. Requirements for joining a SkillsCenter linked lab is the completion and certification of all the lab required skills listed below for each individual lab. Once a student has completed the required skills the SkillsCenter proctors will work with the student and the targeted linked lab to design an independent research project (MCDB 4567) to be completed primarily in the Skills Center. Successful completion of an independent project helps students become eligible to enter into an Honors Thesis project within the targeted linked lab.

ARNOULT LAB

Bacterial Transformation, Buffers and stock solutions, Crispr/Cas9, DNA Agarose Gels, DNA Mini-Prep, FACS, Fluorescence Microscopy, Immunoblot , IP, PCR

EICHEL LAB

Crispr/Cas9 gene editing, DNA Agarose Gel Electrophoresis, Fluorescence Microscopy, Bacterial Transformation, Buffers and stock solutions, DNA Mini-Prep, Centrifugation 1, Pipette Operation and Calibration, BLAST search tool, PCR 1, PCR 2, Advanced FIJI(ImageJ), Autoclave LB/Agar Sterilization.

BETTERTON LAB

Introduction to Chimera, Macromolecule Structure Analysis with Chimera, Autoclave LB/Agar Sterilization, Bacterial Transformation, Buffers and stock solutions, Gene Cloning, Light Microscopy 3 (Fluorescence), PCR, Pipette Operation and Calibration, SDS-PAGE

CECH LAB

Protein Data Bank (PDB) 1, Bacterial Transformation, Buffers and stock solutions, Centrifugation 1, DNA Agarose Gels, DNA Mini-Prep, western blot, PCR, Pipette Operation and Calibration, SDS-PAGE

DOMBROVSKI LAB

PCR 1/PCR 2, Crispr/Cas9, Molecular cloning, Bacterial transformation, DNA agarose gel electrophoresis, DNA miniprep, Making buffers. Transcriptomics Data Analysis, Genomic Data Analysis, Centrifugation 1, Uniprot Database, Light microscopy fluorescence. Autoclave LB/Agar Sterilization, Introduction to FIJI(ImageJ)

LUGER LAB

PCR, Protein Expression, Protein Purification, EM, Cloning, Immunoblot, qPCR, bacterial culture, SDS-PAGE, DNA purification, western blot, protein binding assays, PDB, Uniprot

SHEN LAB

PCR, Protein Purification, Centrifugation, Cloning, FACS, DNA Agarose gels

STOWELL LAB

PCR, Protein Expression, Protein Purification, EM, Cloning, western blot, qPCR, Chimera, Allen Brain Atlas, Chimera, PDB

THINK BIOSCIENCE

PCR. Cloning, Western Blot Immunoprecipitation, Protein Purification, Cell Culture

BIOLOOMICS

Protein Expression, Protein Purification, Cloning, Immunoblot, qPCR, bacterial culture, SDS-PAGE, western blot, protein binding assays

VORTEX BIOTECHNOLOGY

Cloning, bacterial transformation, protein expression, SDS-PAGE, western blotting, protein purification, solutions and media preparation, tissue culture, plate reader assays, PDB, ChimeraX

AMIDEBIO

Cloning, bacterial transformation, protein expression, SDS-PAGE, ITC, MST, protein purification, tissue culture, protein quantification, PDB, Chimera